Mutate for Digest

Format Conversion

-Combine FASTA
-EMBL to FASTA
-EMBL Feature Extractor
-EMBL Trans Extractor
-Filter DNA
-Filter Protein
-GenBank to FASTA
-GenBank Feature Extractor
-GenBank Trans Extractor
-One to Three
-Range Extractor DNA
-Range Extractor Protein
-Reverse Complement
-Split Codons
-Split FASTA
-Three to One
-Window Extractor DNA
-Window Extractor Protein

Sequence Analysis

-Codon Plot
-Codon Usage
-CpG Islands
-DNA Molecular Weight
-DNA Pattern Find
-DNA Stats
-Fuzzy Search DNA
-Fuzzy Search Protein
-Ident and Sim
-Multi Rev Trans
-Mutate for Digest
-ORF Finder
-Pairwise Align Codons
-Pairwise Align DNA
-Pairwise Align Protein
-PCR Primer Stats
-PCR Products
-Protein GRAVY
-Protein Isoelectric Point
-Protein Molecular Weight
-Protein Pattern Find
-Protein Stats
-Restriction Digest
-Restriction Summary
-Reverse Translate
-Translate

Sequence Figures

-Color Align Conservation
-Color Align Properties
-Group DNA
-Group Protein
-Primer Map
-Restriction Map
-Translation Map

Random Sequences

-Mutate DNA
-Mutate Protein
-Random Coding DNA
-Random DNA Sequence
-Random DNA Regions
-Random Protein Sequence
-Random Protein Regions
-Sample DNA
-Sample Protein
-Shuffle DNA
-Shuffle Protein

Miscellaneous

-IUPAC codes
-Genetic codes
-Browser compatibility
-Mirror this site
-Use this site off-line
-About this site
-Acknowledgments
-Reference

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Sequence Manipulation Suite:

Mutate for Digest

Mutate for Digest accepts a DNA sequence as input and searches for regions that can easily be mutated to create a restriction site of interest. The program also displays protein translations so that you can see which reading frames are altered by the proposed mutations. Use Mutate for Digest to find sequences that can be converted to a useful restriction site using PCR or site-directed mutagenesis.

Paste a raw sequence or one or more FASTA sequences into the text area below. Input limit is 10,000,000 characters.

>CDS1
atgtctgattcgctaaatcatccatcgagttctacggtgcatgcagatgatggattcgag
ccaccaacatctccggaagacaacaacaaaaaaccgtctttagaacaaattaaacaggaa
agagaagcgttgtttacggatctattcgcagatcgtcgacgaagcgctcgttctgtgatt
gaagaagctttccaaaacgaactcatgagtgctgaaccagtccagccaaacgtgccgaat
ccacattcgattcccattcgtttccgtcatcaaccagttgctggacctgctcatgatgtt
ttcggagacgcggtgcattcaatttttcaaaaaataatgtccagaggagtgaacgcggat
tatagtcattggatgtcatattggatcgcgttgggaatcgacaaaaaaacacaaatgaac
tatcatatgaaaccgttttgcaaagatacttatgcaactgaaggctccttagaagcgaaa
caaacatttactgataaaatcaggtcagctgttgaggaaattatctggaagtccgctgaa
tattgtgatattcttagcgagaagtggacaggaattcatgtgtcggccgaccaactgaaa
ggtcaaagaaataagcaagaagatcgttttgtggcttatccaaatggacaatacatgaat
cgtggacagagtgacatttcacttcttgcggtgttcgatgggcatggcggacacgagtgc
tctcaatatgcagctgctcatttctgggaagcatggtccgatgctcaacatcatcattca
caagatatgaaacttgacgaactcctagaaaaggctctagaaacattggacgaaagaatg
acagtcagaagtgttcgagaatcttggaaaggtggaaccactgctgtctgctgtgctgtt
gatttgaacactaatcaaatcgcatttgcctggcttggagattcaccaggttacatcatg
tcaaacttggagttccgcaaattcactactgaacactccccgtctgacccggaggaatgt
cgacgagtcgaagaagtcggtggccagatttttgtgatcggtggtgagctccgtgtgaat
ggagtactcaacctgacgcgagcactaggagacgtacctggaagaccaatgatatccaac
aaacctgataccttactgaagacgatcgaacctgcggattatcttgttttgttggcctgt
gacgggatttctgacgtcttcaacactagtgatttgtacaatttggttcaggcttttgtc
aatgaatatgacgtagaagattatcacgaacttgcacgctacatttgcaatcaagcagtt
tcagctggaagtgctgacaatgtgacagtagttataggtttcctccgtccaccagaagac
gtttggcgtgtaatgaaaacagactcggatgatgaagagagcgagctcgaggaagaagat
gacaatgaatag

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Show bases per line.
Show the translation for reading frame
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